Examination of the xanthosine response on gene expression of mammary epithelial cells using RNA-seq technology.

ABSTRACT:

Background

Xanthosine treatment has been previously reported to increase mammary stem cell population and milk production in cattle and goats. However, the underlying molecular mechanisms associated with the increase in stem cell population and milk production remain unclear.

Methods

Primiparous Beetal goats were assigned to the study. Five days post-partum, one mammary gland of each goat was infused with xanthosine (TRT) twice daily (2×) for 3 days consecutively, and the other gland served as a control (CON). Milk samples from the TRT and CON glands were collected on the 10th day after the last xanthosine infusion and the total RNA was isolated from milk fat globules (MEGs). Total RNA in MFGs was mainly derived from the milk epithelial cells (MECs) as evidenced by expressio

SUBMITTER: Choudhary S 

PROVIDER: S-EPMC6045846 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature