Dynamic Variation and Reversion in the Signature Amino Acids of H7N9 Virus During Human Infection.

ABSTRACT: Background:Signature amino acids of H7N9 influenza A virus play critical roles in human adaption and pathogenesis, but their dynamic variation is unknown during disease development. Methods:We sequentially collected respiratory samples from H7N9 patients at different timepoints and applied next-generation sequencing (NGS) to the whole genome of the H7N9 virus to investigate the variation at signature sites. Results:A total of 11 patients were involved, from whom 29 samples were successfully sequenced, including samples from multiple timepoints in 9 patients. Neuraminidase (NA) R292K, basic polymerase 2 (PB2) E627K, and D701N were the 3 most dynamic mutations. The oseltamivir resistance-related NA R292K mutation was present in 9 samples from 5 patients, including 1 sample obtained before an

SUBMITTER: Zou X 

PROVIDER: S-EPMC6047446 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature