Systemic Loss and Gain of Chromatin Architecture throughout Zebrafish Development.

ABSTRACT: The spatial organization of chromosomes is critical in establishing gene expression programs. We generated in situ Hi-C maps throughout zebrafish development to gain insight into higher-order chromatin organization and dynamics. Zebrafish chromosomes segregate in active and inactive chromatin (A/B compartments), which are further organized into topologically associating domains (TADs). Zebrafish A/B compartments and TADs have genomic features similar to those of their mammalian counterparts, including evolutionary conservation and enrichment of CTCF binding sites at TAD borders. At the earliest time point, when there is no zygotic transcription, the genome is highly structured. After zygotic genome activation (ZGA), the genome loses structural features, which are re-established throughout

SUBMITTER: Kaaij LJT 

PROVIDER: S-EPMC6047509 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature