The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.

ABSTRACT: Calcium influx into cells via plasma membrane protein channels is tightly regulated to maintain cellular homeostasis. Calcium channel proteins in the plasma membrane and endoplasmic reticulum have been linked to cancer, specifically during the epithelial-mesenchymal transition (EMT), a cell state transition process implicated in both cancer cell migration and drug resistance. The transcription factor SNAI1 (SNAIL) is upregulated during EMT and is responsible for gene expression changes associated with EMT, but the calcium channels required for Snai1 expression remain unknown. In this study, we show that blocking store-operated calcium entry (SOCE) with 2-aminoethoxydiphenylborane (2APB) reduces cell migration but, paradoxically, increases the level of TGF-β dependent Snai1 ge

SUBMITTER: Bhattacharya A 

PROVIDER: S-EPMC6047677 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature