Amino acids in a targeted versus a non-targeted metabolomics LC-MS/MS assay. Are the results consistent?

ABSTRACT:

Background

The results of plasma amino acid patterns in samples from kidney transplant patients with good and impaired renal function using a targeted LC-MS/MS amino acid assay and a non-targeted metabolomics assay were compared.

Methods

EDTA plasma samples were prospectively collected at baseline, 1, 2, 4 and 6months post-transplant (n=116 patients, n=398 samples). Each sample was analyzed using both a commercial amino acid LC-MS/MS assay and a non-targeted metabolomics assay also based on MS/MS ion transitions. The results of both assays were independently statistically analyzed to identify amino acids associated with estimated glomerular filtration rates using correlation and partial least squares-discriminant analysis.

Results

Although there was overlap between th

SUBMITTER: Klepacki J 

PROVIDER: S-EPMC6047903 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature