O₂-inducible H₂O₂-forming NADPH oxidase is responsible for the hyper O₂ sensitivity of Bifidobacterium longum subsp. infantis.

ABSTRACT: Bifidobacteria are beneficial anaerobes, and their O₂ sensitivity levels differ among species as a function of unknown molecular mechanisms. Bifidobacterium longum subspecies infantis (B. infantis), a predominant colonizer of the gastrointestinal tract of infants, showed a hyper O₂-sensitive growth profile with accompanying a production of H₂O₂. In this study, we characterized an NADPH oxidase as a key enzyme responsible for this microbe's hyper O₂ sensitivity. A dominant active elution peak of H₂O₂-forming NADPH oxidase activity was detected in the first step of column chromatography, and the purified NADPH oxidase (NPOX) was identified as a homolog of nitroreductase family proteins. The introduction of the gene

SUBMITTER: Tanaka K 

PROVIDER: S-EPMC6048055 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature