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Highly efficient and precise base editing by engineered dCas9-guide tRNA adenosine deaminase in rats.

ABSTRACT:

SUBMITTER: Ma Y

PROVIDER: S-EPMC6048098 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Highly efficient and precise base editing by engineered dCas9-guide tRNA adenosine deaminase in rats

Project description:Rats are reference laboratory animal models for understanding human diseases. Engineered point mutation rat model is a powerful tool for understanding the relationship between single nucleotide polymorphisms (SNPs) and human diseases. The newly developed adenine base editor (ABE), fused a modified tRNA adenosine deaminase with a catalytically impaired Cas9 mutant, showed a powerful tool for base editing in target genome sequence. Together with cytosine base editor (CBE), ABE enable the introduction of all four nucleotides transitions (C to T, A to G, T to C, and G to A) in target genomic sequence. Here, we report this new developed base editing tool for application in rat genome base editing with high efficiency even in a multiple method.

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