Acidosis and Phosphate Directly Reduce Myosin's Force-Generating Capacity Through Distinct Molecular Mechanisms.

ABSTRACT: Elevated levels of the metabolic by-products, including acidosis (i.e., high [H⁺]) and phosphate (P_i) are putative agents of muscle fatigue; however, the mechanism through which they affect myosin's function remain unclear. To elucidate these mechanisms, we directly examined the effect of acidosis (pH 6.5 vs. 7.4), alone and in combination with elevated levels of P_i on the force-generating capacity of a mini-ensemble of myosin using a laser trap assay. Acidosis decreased myosin's average force-generating capacity by 20% (p < 0.05). The reduction was due to both a decrease in the force generated during each actomyosin interaction, as well as an increase in the number of binding events generating negative forces. Adding P_i to the acidic cond

SUBMITTER: Woodward M 

PROVIDER: S-EPMC6048269 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature