Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases.

ABSTRACT:

Background

Softwood is a promising feedstock for lignocellulosic biorefineries, but as it contains galactoglucomannan efficient mannan-degrading enzymes are required to unlock its full potential.

Results

Boosting of the saccharification of pretreated softwood (Canadian lodgepole pine) was investigated for 10 fungal endo-β(1→4)-mannanases (endomannanases) from GH5 and GH26, including 6 novel GH26 enzymes. The endomannanases from Trichoderma reesei (TresMan5A) and Podospora anserina (PansMan26) were investigated with and without their carbohydrate-binding module (CBM). The pH optimum and initial rates of enzyme catalysed hydrolysis were determined on pure β-mannans, including acetylated and deacetylated spruce galactoglucomannan. Melting temp

SUBMITTER: von Freiesleben P 

PROVIDER: S-EPMC6048861 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature