KREAP: an automated Galaxy platform to quantify in vitro re-epithelialization kinetics.

ABSTRACT:

Background

In vitro scratch assays have been widely used to study the influence of bioactive substances on the processes of cell migration and proliferation that are involved in re-epithelialization. The development of high-throughput microscopy and image analysis has enabled scratch assays to become compatible with high-throughput research. However, effective processing and in-depth analysis of such high-throughput image datasets are far from trivial and require integration of multiple image processing and data extraction software tools.

Findings

We developed and implemented a kinetic re-epithelialization analysis pipeline (KREAP) in Galaxy. The KREAP toolbox incorporates freely available image analysis tools and automatically performs image segmentation and feature extract

SUBMITTER: Fernandez-Gutierrez MM 

PROVIDER: S-EPMC6048990 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature