DNA methylation of amino acid transporter genes in the human placenta.

ABSTRACT:

Introduction

Placental transfer of amino acids via amino acid transporters is essential for fetal growth. Little is known about the epigenetic regulation of amino acid transporters in placenta. This study investigates the DNA methylation status of amino acid transporters and their expression across gestation in human placenta.

Methods

BeWo cells were treated with 5-aza-2'-deoxycytidine to inhibit methylation and assess the effects on amino acid transporter gene expression. The DNA methylation levels of amino acid transporter genes in human placenta were determined across gestation using DNA methylation array data. Placental amino acid transporter gene expression across gestation was also analysed using data from publically available Gene Expression Omnibus data sets. The exp

SUBMITTER: Simner C 

PROVIDER: S-EPMC6049612 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature