Evaluation of in vitro neuronal platforms as surrogates for in vivo whole brain systems.

ABSTRACT: Quantitatively benchmarking similarities and differences between the in vivo central nervous system and in vitro neuronal cultures can qualify discrepancies in functional responses and establish the utility of in vitro platforms. In this work, extracellular electrophysiology responses of cortical neurons in awake, freely-moving animals were compared to in vitro cultures of dissociated cortical neurons. After exposure to two well-characterized drugs, atropine and ketamine, a number of key points were observed: (1) significant differences in spontaneous firing activity for in vivo and in vitro systems, (2) similar response trends in single-unit spiking activity after exposure to atropine, and (3) greater sensitivity to the effects of ketamine in vitro. While in vitro cultures of dissociated

SUBMITTER: Belle AM 

PROVIDER: S-EPMC6050270 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature