Calibration of force detection for arbitrarily shaped particles in optical tweezers.

ABSTRACT: Force measurement with an optical trap requires calibration of it. With a suitable detector, such as a position-sensitive detector (PSD), it is possible to calibrate the detector so that the force can be measured for arbitrary particles and arbitrary beams without further calibration; such a calibration can be called an "absolute calibration". Here, we present a simple method for the absolute calibration of a PSD. Very often, paired position and force measurements are required, and even if synchronous measurements are possible with the position and force detectors used, knowledge of the force-position curve for the particle in the trap can be highly beneficial. Therefore, we experimentally demonstrate methods for determining the force-position curve with and without synchronous force and p

SUBMITTER: Bui AAM 

PROVIDER: S-EPMC6050307 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature