Application of Hanging Drop Technique for Kidney Tissue Culture.

ABSTRACT:

Background/aims

The hanging drop technique is a well-established method used in culture of animal tissues. However, this method has not been used in adult kidney tissue culture yet. This study was to explore the feasibility of using this technique for culturing adult kidney cortex to study the time course of RNA viability in the tubules and vasculature, as well as the tissue structural integrity.

Methods

In each Petri dish with the plate covered with sterile buffer, a section of mouse renal cortex was cultured within a drop of DMEM culture medium on the inner surface of the lip facing downward. The tissue were then harvested at each specific time points for Real-time PCR analysis and histological studies.

Results

The results showed that the mRNA level of most Na+ rela

SUBMITTER: Wang S 

PROVIDER: S-EPMC6050513 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature