Cryptophane Nanoscale Assemblies Expand ¹²⁹Xe NMR Biosensing.

ABSTRACT: Cryptophane-based biosensors are promising agents for the ultrasensitive detection of biomedically relevant targets via ¹²⁹Xe NMR. Dynamic light scattering revealed that cryptophanes form water-soluble aggregates tens to hundreds of nanometers in size. Acridine orange fluorescence quenching assays allowed quantitation of the aggregation state, with critical concentrations ranging from 200 nM to 600 nM, depending on the cryptophane species in solution. The addition of excess carbonic anhydrase (CA) protein target to a benzenesulfonamide-functionalized cryptophane biosensor (C8B) led to C8B disaggregation and produced the expected 1:1 C8B-CA complex. C8B showed higher affinity at 298 K for the cytoplasmic isozyme CAII than the extracellular CAXII isozyme, which is a biomarker of c

SUBMITTER: Zemerov SD 

PROVIDER: S-EPMC6050516 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature