Heterologous production of levopimaric acid in Saccharomyces cerevisiae.

ABSTRACT:

Background

Levopimaric acid (LA), a type of diterpene resin acid produced by plants, is a significant industrial intermediate that is mainly produced via phytoextraction. This work aimed to apply synthetic biology to produce LA in yeast strains from a simple carbon source.

Results

Levopimaradiene (LP), the precursor of LA, was produced via LP synthase (LPS) expression in yeast. LPS was then modified by N-terminal truncating and site-directed mutagenesis. The strain containing t79LPS^MM (79 N-terminal amino acid truncating and M593I/Y700F mutation) produced 6.92 mg/L of LP, which were 23-fold higher than the strain containing LPS. Next, t79LPS^MM was expressed in a new metabolically engineered chassis, and the final LP production increased 164-folds to 49.

SUBMITTER: Liu T 

PROVIDER: S-EPMC6050663 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature