Persistent presence of outer membrane epitopes during short- and long-term starvation of five Legionella pneumophila strains.

ABSTRACT:

Background

Legionella pneumophila, the causative agent of Legionnaire's disease, may enter a viable but non-culturable (VBNC) state triggered by environmental stress conditions. Specific outer-membrane epitopes of L. pneumophila are used in many diagnostic applications and some of them are linked to important virulence-related factors or endotoxins. However, it is not clear how the presence and status of these epitopes are influenced by environmental stress conditions. In this study, changes of outer membrane epitopes for monoclonal antibodies (mAb) from the Dresden panel and the major outer membrane protein MOMP were analysed for five L. pneumophila strains during short- and long-term starvation in ultrapure water.

Results

With ELISA and single cell immuno-fluorescence anal

SUBMITTER: Schrammel B 

PROVIDER: S-EPMC6050704 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature