A Multicolor Split-Fluorescent Protein Approach to Visualize Listeria Protein Secretion in Infection.

ABSTRACT: Listeria monocytogenes is an intracellular food-borne pathogen that has evolved to enter mammalian host cells, survive within them, spread from cell to cell, and disseminate throughout the body. A series of secreted virulence proteins from Listeria are responsible for manipulation of host-cell defense mechanisms and adaptation to the intracellular lifestyle. Identifying when and where these virulence proteins are located in live cells over the course of Listeria infection can provide valuable information on the roles these proteins play in defining the host-pathogen interface. These dynamics and protein levels may vary from cell to cell, as bacterial infection is a heterogeneous process both temporally and spatially. No assay to visualize virulence proteins over time in infection with List

SUBMITTER: Batan D 

PROVIDER: S-EPMC6050711 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature