Thrombopoietin knock-in augments platelet generation from human embryonic stem cells.

ABSTRACT:

Background

Refinement of therapeutic-scale platelet production in vitro will provide a new source for transfusion in patients undergoing chemotherapy or radiotherapy. However, procedures for cost-effective and scalable platelet generation remain to be established.

Methods

In this study, we established human embryonic stem cell (hESC) lines containing knock-in of thrombopoietin (TPO) via CRISPR/Cas9-mediated genome editing. The expression and secretion of TPO was detected by western blotting and enzyme-linked immunosorbent assay. Then, we tested the potency for hematopoietic differentiation by coculturing the cells with mAGM-S3 cells and measured the generation of CD43⁺ and CD45⁺ hematopoietic progenitor cells (HPCs). The potency for megakaryocytic diffe

SUBMITTER: Zhang L 

PROVIDER: S-EPMC6050740 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature