Single Proteoliposome High-Content Analysis Reveals Differences in the Homo-Oligomerization of GPCRs.

ABSTRACT: G-protein-coupled receptors (GPCRs) control vital cellular signaling pathways. GPCR oligomerization is proposed to increase signaling diversity. However, many reports have arrived at disparate conclusions regarding the existence, stability, and stoichiometry of GPCR oligomers, partly because of cellular complexity and ensemble averaging of intrareconstitution heterogeneities that complicate the interpretation of oligomerization data. To overcome these limitations, we exploited fluorescence-microscopy-based high-content analysis of single proteoliposomes. This allowed multidimensional quantification of intrinsic monomer-monomer interactions of three class A GPCRs (β₂-adrenergic receptor, cannabinoid receptor type 1, and opsin). Using a billion-fold less protein than conventional

SUBMITTER: Walsh SM 

PROVIDER: S-EPMC6050755 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature