DNA methylation changes in CD4⁺ T cells isolated from multiple sclerosis patients on dimethyl fumarate.

ABSTRACT:

Background

Dimethyl fumarate is an oral treatment for multiple sclerosis, whose mechanism of action is not fully understood.

Objective

To investigate the effects of dimethyl fumarate on DNA methylation in the CD4⁺ T cells of multiple sclerosis patients.

Methods

We performed Illumina EPIC arrays to investigate the DNA methylation profiles of CD4⁺ T cells derived from multiple sclerosis patients before and after dimethyl fumarate treatment.

Results

Treatment with dimethyl fumarate resulted in 97% of differentially methylated positions showing hypermethylation. Four genes, SNORD1A, SHTN1, MZB1 and TNF had a differentially methylated region located within the transcriptional start site.

Conclusion

This study

SUBMITTER: Maltby VE 

PROVIDER: S-EPMC6050818 | biostudies-literature | 2018 Jul-Sep

REPOSITORIES: biostudies-literature