Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen.

ABSTRACT: The microenvironment is an important factor of stem cells regulating their maintenance, survival, and differentiation. The glycation of proteins with reducing sugars through nonenzymatic reactions induces the collagen cross-linking, which causes tissue stiffening, which is enhanced during aging and diabetes. In this study, we aimed to analyze the effect of glycated collagen on the stem cell culture and differentiation. The collagen type 1 was modified by glycation with mannose, rhamnose, arabinose, and glucose. After the culture of mesenchymal stem cells on the coated surfaces with glycated collagen, the differences in cell adhesion, proliferation, and differentiation were compared. The results showed that the modifications did not induce apoptosis or cause cell death. However, the culture

SUBMITTER: Duruksu G 

PROVIDER: S-EPMC6051021 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature