Microfluidics-based super-resolution microscopy enables nanoscopic characterization of blood stem cell rolling.

ABSTRACT: Hematopoietic stem/progenitor cell (HSPC) homing occurs via cell adhesion mediated by spatiotemporally organized ligand-receptor interactions. Although molecules and biological processes involved in this multistep cellular interaction with endothelium have been studied extensively, molecular mechanisms of this process, in particular the nanoscale spatiotemporal behavior of ligand-receptor interactions and their role in the cellular interaction, remain elusive. We introduce a microfluidics-based super-resolution fluorescence imaging platform and apply the method to investigate the initial essential step in the homing, tethering, and rolling of HSPCs under external shear stress that is mediated by selectins, expressed on endothelium, with selectin ligands (that is, CD44) expressed on HSPCs.

SUBMITTER: AbuZineh K 

PROVIDER: S-EPMC6051739 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature