Covalent labeling-mass spectrometry with non-specific reagents for studying protein structure and interactions.

ABSTRACT: Using mass spectrometry (MS) to obtain information about a higher order structure of protein requires that a protein's structural properties are encoded into the mass of that protein. Covalent labeling (CL) with reagents that can irreversibly modify solvent accessible amino acid side chains is an effective way to encode structural information into the mass of a protein, as this information can be read-out in a straightforward manner using standard MS-based proteomics techniques. The differential reactivity of proteins under two or more conditions can be used to distinguish protein topologies, conformations, and/or binding sites. CL-MS methods have been effectively used for the structural analysis of proteins and protein complexes, particularly for systems that are difficult to study by oth

SUBMITTER: Limpikirati P 

PROVIDER: S-EPMC6051898 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature