A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA.

ABSTRACT: The methylotrophic yeast Pichia pastoris is widely used to produce recombinant proteins, taking advantage of this species' high-density cell growth and strong ability to secrete proteins. Circular plasmids containing the P. pastoris-specific autonomously replicating sequence (PARS1) permit transformation of P. pastoris with higher efficiency than obtained following chromosomal integration by linearized DNA. Unfortunately, however, existing autonomously replicating plasmids are known to be inherently unstable. In this study, we used transcriptome sequencing (RNA-seq) data and genome sequence information to independently identify, on each of the four chromosomes, centromeric DNA sequences consisting of long inverted repeat sequences. By examining the chromosome 2 centromeric DNA sequence (Ce

SUBMITTER: Nakamura Y 

PROVIDER: S-EPMC6052274 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature