Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells.

ABSTRACT:

Background

Male germline stem cells (mGSCs) offer great promise in regenerative medicine and animal breeding due to their capacity to maintain self-renewal and to transmit genetic information to the next generation following spermatogenesis. Human testis-derived embryonic stem cell-like cells have been shown to possess potential of mesenchymal progenitors, but there remains confusion about the characteristics and origin of porcine testis-derived stem cells.

Methods

Porcine testis-derived stem cells were obtained from primary testicular cultures of 5-day old piglets, and selectively expanded using culture conditions for long-term culture and induction differentiation. The stem cell properties of porcine testis-derived stem cells were subsequently assessed by determining the e

SUBMITTER: Bai Y 

PROVIDER: S-EPMC6052628 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature