Design of a ratiometric two-photon probe for imaging of hypochlorous acid (HClO) in wounded tissues.

ABSTRACT: HClO plays crucial roles in a wide range of biological and pathological processes. Recent studies have revealed that the generation of HClO has close links with the wound healing process. It's thus meaningful to develop a reliable method for monitoring HClO in wounded tissues. Toward this purpose, we herein report a rationally designed quinolone-based ratiometric two-photon fluorescent probe, QClO, for HClO. The probe QClO rapidly displays a drop in blue emission and an increase of green emission in response to HClO due to the oxidation of oxathiolane. The fluorescence intensity ratio (green/blue) can serve as the ratiometric detection signal for HClO with high sensitivity. After confirming its excellent sensing performance in vitro, the probe was validated by detectin

SUBMITTER: Mao Z 

PROVIDER: S-EPMC6052737 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature