Process for production and purification of Lethal Toxin Neutralizing Factor (LTNF) from E. coli and its economic analysis.

ABSTRACT:

Background

Purification of peptides offers unique challenges with respect to obtaining the desired process yield and selectivity. Lethal Toxin Neutralizing Factor (LTNF) is a peptide that is known to neutralize snake venom in mice when the peptide is preincubated with the venom prior to intravenous injection. A process for producing highly purified recombinant LTNF has been developed. The process has been modelled in SuperPro designer using laboratory data for a plant capable of producing 10 Kg of purified rLTNF. Economic analysis has been performed for manufacturing 3 ton of purified rLTNF.

Results

The process developed produces peptide in the form of concatemer that has been specifically designed to accumulate as insoluble inclusion bodies (IB) during expression in E. c

SUBMITTER: Hebbi V 

PROVIDER: S-EPMC6052786 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature