The unique biomineralization transcriptome and proteome of Lytechinus variegatus teeth.

ABSTRACT: BACKGROUND:Matrix-regulated biomineralization involves the specific nucleation and growth of mineral phases within or upon preformed structured organic matrices. We hypothesized that there might be a general mechanism whereby anionic, phosphorylated mineral ion-binding proteins assist in specifically locating the mineral ions with respect to the mineralizing structural organic matrix. Here we extended these studies to invertebrate mineralization in Lytechinus variegatus (Lv) teeth. MATERIALS AND METHODS:The tooth proteins were extracted and the phosphoproteins occluded in the mineral were enriched by passage through a ProQ Diamond phosphoprotein enrichment column, and subjected to MS/MS analysis. A Lv RNA-seq derived transcriptome database was generated. The MS/MS data found 25 proteins pr

SUBMITTER: Alvares K 

PROVIDER: S-EPMC6052879 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature