Quantification of microRNA levels in plasma - Impact of preanalytical and analytical conditions.

ABSTRACT: Numerous studies have reported a potential role for circulating microRNAs as biomarkers in a wide variety of diseases. However, there is a critical reproducibility challenge some of which might be due to differences in preanalytical and/or analytical factors. Thus, in the current study we systematically investigated the impact of selected preanalytical and analytical variables on the measured microRNA levels in plasma. Similar levels of microRNA were found in platelet-poor plasma obtained by dual compared to prolonged single centrifugation. In contrast, poor correlation was observed between measurements in standard plasma compared to platelet-poor plasma. The correlation between quantitative real-time PCR and droplet digital PCR was found to be good, contrary to TaqMan Low Density Array an

SUBMITTER: Binderup HG 

PROVIDER: S-EPMC6053236 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature