Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.

ABSTRACT: Compartmentalized partnered replication (CPR) is an emulsion-based directed evolution method based on a robust and modular phenotype-genotype linkage. In contrast to other in vivo directed evolution approaches, CPR largely mitigates host fitness effects due to a relatively short expression time of the gene of interest. CPR is based on gene circuits in which the selection of a 'partner' function from a library leads to the production of a thermostable polymerase. After library preparation, bacteria produce partner proteins that can potentially lead to enhancement of transcription, translation, gene regulation, and other aspects of cellular metabolism that reinforce thermostable polymerase production. Individual cells are then trapped in water-in-oil emulsion droplets in the presence of prim

SUBMITTER: Abil Z 

PROVIDER: S-EPMC6053311 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature