SLAM-ITseq: sequencing cell type-specific transcriptomes without cell sorting.

ABSTRACT: Cell type-specific transcriptome analysis is an essential tool for understanding biological processes in which diverse types of cells are involved. Although cell isolation methods such as fluorescence-activated cell sorting (FACS) in combination with transcriptome analysis have widely been used so far, their time-consuming and harsh procedures limit their applications. Here, we report a novel in vivo metabolic RNA sequencing method, SLAM-ITseq, which metabolically labels RNA with 4-thiouracil in a specific cell type in vivo followed by detection through an RNA-seq-based method that specifically distinguishes the thiolated uridine by base conversion. This method has successfully identified the cell type-specific transcriptome in three different tissues: endothelial cells in br

SUBMITTER: Matsushima W 

PROVIDER: S-EPMC6053661 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature