Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling.

ABSTRACT: Upon gastrulation, the mammalian conceptus transforms rapidly from a simple bilayer into a multilayered embryo enveloped by its extra-embryonic membranes. Impaired development of the amnion, the innermost membrane, causes major malformations. To clarify the origin of the mouse amnion, we used single-cell labelling and clonal analysis. We identified four clone types with distinct clonal growth patterns in amniotic ectoderm. Two main types have progenitors in extreme proximal-anterior epiblast. Early descendants initiate and expand amniotic ectoderm posteriorly, while descendants of cells remaining anteriorly later expand amniotic ectoderm from its anterior side. Amniogenesis is abnormal in embryos deficient in the bone morphogenetic protein (BMP) signalling effector SMAD5, with delayed clos

SUBMITTER: Dobreva MP 

PROVIDER: S-EPMC6053664 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature