High-Throughput Investigation of Diverse Junction Elements in RNA Tertiary Folding.

ABSTRACT: RNAs fold into defined tertiary structures to function in critical biological processes. While quantitative models can predict RNA secondary structure stability, we are still unable to predict the thermodynamic stability of RNA tertiary structure. Here, we probe conformational preferences of diverse RNA two-way junctions to develop a predictive model for the formation of RNA tertiary structure. We quantitatively measured tertiary assembly energetics of >1,000 of RNA junctions inserted in multiple structural scaffolds to generate a "thermodynamic fingerprint" for each junction. Thermodynamic fingerprints enabled comparison of junction conformational preferences, revealing principles for how sequence influences 3-dimensional conformations. Utilizing fingerprints of junctions with known cryst

SUBMITTER: Denny SK 

PROVIDER: S-EPMC6053692 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature