Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis.

ABSTRACT:

Background

Necroptotic susceptibility is probably an intrinsic weakness of cancer. Here, we report that resibufogenin, a member of bufadienolide family, suppresses the growth and metastasis of colorectal cancer (CRC) through induction of necroptosis in vivo.

Methods

SW480 cells with stably expressing enhanced green fluorescence protein were xenografted to BALB/c-nu mice to observe the growth of tumors. Liver metastasis was observed by injection of MC38 cells beneath the splenic capsule of mice. Protein expression was determined by immunohistochemistry, immunofluorescence and western blot.

Results

Consolidated in vitro results indicate that resibufogenin has anti-proliferative activity on CRC cells. PI staining and transmission electron microscope imaging suggest that

SUBMITTER: Han Q 

PROVIDER: S-EPMC6053767 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature