A "turn on" fluorescent probe for heparin and its oversulfated chondroitin sulfate contaminant.

ABSTRACT: Designing "turn on" fluorescent probes for heparin (Hep), a widely used anticoagulant in clinics, is of great importance but remains challenging. By introducing a Hep specific binding peptide AG73 to a typical aggregation induced emission (AIE) fluorogen, tetraphenylethene (TPE), a sensitive and selective "turn on" fluorescent probe named TPE-1 for Hep was developed. TPE-1 was able to detect Hep in a wide pH range of 3-10 without obvious interference from tested anions and biomolecules, especially Hep analogues known as chondroitin sulfate (Chs) and hyaluronic acid (HA). The detection limit of Hep sensing was 3.8 ng mL^-1, which was far below the clinically demanded concentration of Hep. The probe was applicable to both unfractionated Hep and low molecular weight He

SUBMITTER: Ding Y 

PROVIDER: S-EPMC6054120 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature