Immune cell census in murine atherosclerosis: cytometry by time of flight illuminates vascular myeloid cell diversity.

ABSTRACT:

Aims

Atherosclerosis is characterized by the abundant infiltration of myeloid cells starting at early stages of disease. Myeloid cells are key players in vascular immunity during atherogenesis. However, the subsets of vascular myeloid cells have eluded resolution due to shared marker expression and atypical heterogeneity in vascular tissues. We applied the high-dimensionality of mass cytometry to the study of myeloid cell subsets in atherosclerosis.

Methods and results

Apolipoprotein E-deficient (ApoE-/-) mice were fed a chow or a high fat (western) diet for 12 weeks. Single-cell aortic preparations were probed with a panel of 35 metal-conjugated antibodies using cytometry by time of flight (CyTOF). Clustering of marker expression on live CD45+ cells from the aortas of ApoE-

SUBMITTER: Cole JE 

PROVIDER: S-EPMC6054192 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature