Hypoglycemic activity and constituents analysis of blueberry (Vaccinium corymbosum) fruit extracts.

ABSTRACT:

Background

To investigate hypoglycemic activity and elucidate the active composition of the fruit blueberry (Vaccinium corymbosum).

Methods

Methanol extracts of blueberry (MEB) were separated using a D101 macroporous resin column to yield quinic acid derivative (Fr.1)- and flavonoid (Fr.2)-rich fractions. The effects of the blueberry extracts on mRNA expression of GLUT-2 (glucose transporter type 2) and PPARγ (peroxisome proliferator-activated receptor-γ), as well as on the activities of PPRE (peroxisome proliferator response element) and NF-κB were analyzed in LO2 normal liver cells. Real-time PCR was used to detect the expression of GLUT-2, PPARγ, TNF-α, IL-1β, and IL-6 mRNA. The PPRE and NF-κB activities were detected by a luciferase reporter assay. Western blottin

SUBMITTER: Huang W 

PROVIDER: S-EPMC6054273 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature