Acid Stimulation of the Citrate Transporter NaDC-1 Requires Pyk2 and ERK1/2 Signaling Pathways.

ABSTRACT: Background Urine citrate is reabsorbed exclusively along the renal proximal tubule via the apical Na⁺-dicarboxylate cotransporter NaDC-1. We previously showed that an acid load in vivo and media acidification in vitro increase NaDC-1 activity through endothelin-1 (ET-1)/endothelin B (ET_B) signaling. Here, we further examined the signaling pathway mediating acid-induced NaDC-1 activity.Methods We transiently transfected cultured opossum kidney cells, a model of the proximal tubule, with NaDC-1 and ET_B and measured [¹⁴C]-citrate uptake after media acidification under various experimental conditions, including inactivation of Pyk2 and c-Src, which were previously shown to be activated by media acidification. Wild-t

SUBMITTER: Zacchia M 

PROVIDER: S-EPMC6054333 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature