An Automated Multidose Synthesis of the Potentiometric PET Probe 4-[¹⁸F]Fluorobenzyl-Triphenylphosphonium ([¹⁸F]FBnTP).

ABSTRACT:

Purpose

The aim of this study was the automated synthesis of the mitochondrial membrane potential sensor 4-[¹⁸F]fluorobenzyl-triphenylphosphonium ([¹⁸F]FBnTP) on a commercially available synthesizer in activity yields (AY) that allow for imaging of multiple patients.

Procedures

A three-pot, four-step synthesis was implemented on the ELIXYS FLEX/CHEM radiosynthesizer (Sofie Biosciences) and optimized for radiochemical yield (RCY), radiochemical purity (RCP) as well as chemical purity during several production runs (n = 24). The compound was purified by solid-phase extraction (SPE) with a Sep-Pak Plus Accell CM cartridge, thereby avoiding HPLC purification.

Results

Under optimized conditions, AY of 1.4-2.2 GBq of [¹⁸F]FBnTP were obtained

SUBMITTER: Waldmann CM 

PROVIDER: S-EPMC6054454 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature