Chemiluminescent Biosensors for Detection of Second Messenger Cyclic di-GMP.

ABSTRACT: Bacteria colonize highly diverse and complex environments, from gastrointestinal tracts to soil and plant surfaces. This colonization process is controlled in part by the intracellular signal cyclic di-GMP, which regulates bacterial motility and biofilm formation. To interrogate cyclic di-GMP signaling networks, a variety of fluorescent biosensors for live cell imaging of cyclic di-GMP have been developed. However, the need for external illumination precludes the use of these tools for imaging bacteria in their natural environments, including in deep tissues of whole organisms and in samples that are highly autofluorescent or photosensitive. The need for genetic encoding also complicates the analysis of clinical isolates and environmental samples. Toward expanding the study of bacterial si

SUBMITTER: Dippel AB 

PROVIDER: S-EPMC6054565 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature