A simple diet- and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer.

ABSTRACT:

Background and aims

Although the majority of patients with non-alcoholic fatty liver disease (NAFLD) have only steatosis without progression, a sizeable fraction develop non-alcoholic steatohepatitis (NASH), which can lead to cirrhosis and hepatocellular carcinoma (HCC). Many established diet-induced mouse models for NASH require 24-52 weeks, which makes testing for drug response costly and time consuming.

Methods

We have sought to establish a murine NASH model with rapid progression of extensive fibrosis and HCC by using a western diet (WD), which is high-fat, high-fructose and high-cholesterol, combined with low weekly dose of intraperitoneal carbon tetrachloride (CCl₄), which serves as an accelerator.

Results

C57BL/6J mice were fed a normal chow diet ± C

SUBMITTER: Tsuchida T 

PROVIDER: S-EPMC6054570 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature