Withdrawal From Cocaine Self-administration Alters the Regulation of Protein Translation in the Nucleus Accumbens.

ABSTRACT:

Background

Cue-induced cocaine craving incubates during abstinence from cocaine self-administration. Expression of incubation ultimately depends on elevation of homomeric GluA1 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors in the nucleus accumbens (NAc). This adaptation requires ongoing protein translation for its maintenance. Aberrant translation is implicated in central nervous system diseases, but nothing is known about glutamatergic regulation of translation in the drug-naïve NAc or after incubation.

Methods

NAc tissue was obtained from drug-naïve rats and from rats after 1 or >40 days of abstinence from extended-access cocaine or saline self-administration. Newly translated proteins were labeled using ³⁵S-Met/Cys or puromycin. We compare

SUBMITTER: Stefanik MT 

PROVIDER: S-EPMC6054574 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature