Integrated mRNA, sRNA, and degradome sequencing reveal oilseed rape complex responses to Sclerotinia sclerotiorum (Lib.) infection.

ABSTRACT: Sclerotinia stem rot (SSR), caused by the fungal pathogen Sclerotinia sclerotiorum, is a devastating disease resulting in yield losses and decreases in seed quality in oilseed rape (Brassica napus) worldwide. However, the molecular mechanisms underlying the response of oilseed rape to S. sclerotiorum infection at the transcriptional and post-transcriptional levels are poorly understood. Here, we used an integrated omics approach (transcriptome, sRNAome, and degradome sequencing) on the Illumina platform to compare the RNA expression and post-transcriptional profiles of oilseed rape plants inoculated or not with S. sclerotiorum. In total, 7,065 differentially expressed genes (DEGs) compared with the mock-inoculated control at 48 hours post inoculation were identified. These DEGs were associ

SUBMITTER: Jian H 

PROVIDER: S-EPMC6054686 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature