Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin.

ABSTRACT:

Background

Cis, cis-muconic acid (MA) is a dicarboxylic acid of recognized industrial value. It provides direct access to adipic acid and terephthalic acid, prominent monomers of commercial plastics.

Results

In the present work, we engineered the soil bacterium Corynebacterium glutamicum into a stable genome-based cell factory for high-level production of bio-based MA from aromatics and lignin hydrolysates. The elimination of muconate cycloisomerase (catB) in the catechol branch of the β-ketoadipate pathway provided a mutant, which accumulated MA at 100% molar yield from catechol, phenol, and benzoic acid, using glucose as additional growth substrate. The production of MA was optimized by constitutive overexpression of catA, which increased the activity of the encoded catech

SUBMITTER: Becker J 

PROVIDER: S-EPMC6054733 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature