Photopolymerized dynamic hydrogels with tunable viscoelastic properties through thioester exchange.

ABSTRACT: The extracellular matrix (ECM) constitutes a viscoelastic environment for cells. A growing body of evidence suggests that the behavior of cells cultured in naturally-derived or synthetic ECM mimics is influenced by the viscoelastic properties of these substrates. Adaptable crosslinking strategies provide a means to capture the viscoelasticity found in native soft tissues. In this work, we present a covalent adaptable hydrogel based on thioester exchange as a biomaterial for the in vitro culture of human mesenchymal stem cells. Through control of pH, gel stoichiometry, and crosslinker structure, viscoelastic properties in these crosslinked networks can be modulated across several orders of magnitude. We also propose a strategy to alter these properties in existing networks by the photo-unca

SUBMITTER: Brown TE 

PROVIDER: S-EPMC6054801 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature