Imaging mass spectrometry reveals direct albumin fragmentation within the diabetic kidney.

ABSTRACT: Albumin degradation in the renal tubules is impaired in diabetic nephropathy such that levels of the resulting albumin fragments increase with the degree of renal injury. However, the mechanism of albumin degradation is unknown. In particular, fragmentation of the endogenous native albumin has not been demonstrated in the kidney and the enzymes that may contribute to fragmentation have not been identified. To explore this we utilized matrix-assisted laser desorption/ionization imaging mass spectrometry for molecular profiling of specific renal regions without disturbing distinct tissue morphology. Changes in protein expression were measured in kidney sections of eNOS^-/-db/db mice, a model of diabetic nephropathy, by high spatial resolution imaging allowing molecular localization

SUBMITTER: Grove KJ 

PROVIDER: S-EPMC6054886 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature