A method package for electrophysiological evaluation of reconstructed or regenerated facial nerves in rodents.

ABSTRACT: Compound muscle action potential (CMAP) recording via reconstructed or regenerated motor axons is a critical examination to evaluate newly developed surgical and regeneration techniques. However, there is currently no documentation on technical aspects of CMAP recordings via reconstructed or regenerated facial nerves. We have studied new techniques of plastic surgery and nerve regeneration using a rat facial nerve defect model for years, standardizing an evaluation pipeline using CMAP recordings. Here we describe our CMAP recording procedure in detail as a package including surgical preparation, data acquisition, analysis and troubleshooting. Each resource is available in public repositories and is maintained as a version control system. In addition, we demonstrate that our analytical pipe

SUBMITTER: Takeuchi Y 

PROVIDER: S-EPMC6055010 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature