TRPM7-mediated spontaneous Ca²⁺ entry regulates the proliferation and differentiation of human leukemia cell line K562.

ABSTRACT: Continuous Ca²⁺ influx is essential to maintain intracellular Ca²⁺ homeostasis and its dysregulation leads to a variety of cellular dysfunctions. In this study, we explored the functional roles of spontaneous Ca²⁺ influx for the proliferation and differentiation of a human erythromyeloid leukemia cell line K562. mRNA/protein expressions were assessed by the real-time RT-PCR, western blotting, and immunocytochemical staining. Intracellular Ca²⁺ concentration ([Ca²⁺ ]_i ) and ionic currents were measured by fluorescent imaging and patch clamping techniques, respectively. Cell counting/viability and colorimetric assays were applied to assess proliferation rate and hemoglobin synthesis, respectively. Elimination of extracellular

SUBMITTER: Takahashi K 

PROVIDER: S-EPMC6055029 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature