Parallel folding topology-selective label-free detection and monitoring of conformational and topological changes of different G-quadruplex DNAs by emission spectral changes via FRET of mPPE-Ala-Pt(ii) complex ensemble.

ABSTRACT: The formation of supramolecular assemblies between [Pt(bzimpy-Et){C[triple bond, length as m-dash]CC₆H₄(CH₂NMe₃-4)}]Cl₂ (1) and mPPE-Ala and the FRET properties of the ensemble have been revealed from the UV-vis absorption, steady-state emission and time-resolved emission decay studies. The two-component mPPE-Ala-1 ensemble has been employed in a "proof-of-principle" concept for label-free detection of G-quadruplex DNAs with the intramolecular propeller parallel folding topology, such as c-myc, in aqueous buffer solution. By the modulation of the aggregation/deaggregation of the polymer-metal complex aggregates and hence the FRET from the mPPE-Ala donor to the aggregated 1 as acceptor, t

SUBMITTER: Chan K 

PROVIDER: S-EPMC6055111 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature